ADVANCE DIAGNOSTIC TECHNIQUES - Mcqs
(1) basic components of a photometry are.......?
(A) A light source
(B) filter
(C) cuvette
(D) All of the above - Ans
(2) which of the following is not an application of colorimeter ?
(A) paints
(B) links
(C) cosmetics
(D) composition detection - Ans
(3) which of the following is a source used in colorimeter
(A) laser
(B) tube light
(C ) sodium vapor lamp
(D) Tungsten lamp - Ans
(4) The device produce monochromatic beam of the light is...
(A) prism
(B) filter - Ans
(C) Diffraction gratting
(D) none
(5) Beer’s law states that the intesity of light decrease with respect to….?
(A) concentration - Ans
(B) Distance
(C) composition
(D) volume
(6) which of the following prism will you use for IR spectroscopy ?
(A) glass prism
(B) fused silica prism
(C) prism coated with sodium chloride - Ans
(D) All can be used
(7) When the entire CBC is suppressed due to either anemia,infection, or hemorrhage is called ?
(A) Erythroplasia
(B) Thrombocytopenia
(C) Pancytopenia - Ans
(D) Leukopenia
(8) which of the following techniques dose not involve nucleic acids?
(A) Northern blotting
(B) Western blotting - Ans
(C) PFGE
(D) Southern blotting
(9) Which is not application of Elisa ?
(A) Detection of hepatitis B markers in serum
(B) Percentage of Hb inblood - Ans
(C) Detection of HIV antibodies in blood sample
(D) Detection of mycobacterium antibodies in tuberculosis
(10) Infrared spectroscopy is the _ type spectroscopy ?
(A) Absorption spectroscopy - Ans
(B) Fluorescence spectroscopy
(C ) Emission spectroscopy
(D) Electron spectroscopy
(11) Elisa allows for rapid screening and qualification of the presence of in a sample ?
(A) Amino acid
(B) DNA
(C) Antigen - Ans
(D)Protein
(12) The Quantum mechanical energy levels observed in IR spectroscopy are those of ?
(A) Atomic vibration
(B) molecular vibration - Ans
(C ) Both of A and B
(D) elemental vibration
(13) What is the role of goat anti-rabbit IgG horseradish peroxidase conjugate in the experiment ?
(A) Antigen
(B) Primaryantibody
(C) second antibody - Ans
(D) Substance
(14) Please arrange the following steps about Elisa in chronological order.
(i) Incubate with antibody -enzyme complex that binds primary antibody.
(ii) Coat surface with antigen block unoccupied sites with nonspecific protein
(iii) Add substance formation of colored product indicates presence of specific antigen
(iv) Incubate with primary antibody against specific antigen
(A) I, iv, ii, iii
(B) I,iv,iii,ii
(C)ii, iv, iii, i
(D) ii, iv, i, iii - Ans
(25) Which of the following are commonly tested for using the Elisa method ?
(A) HIV
(B) Pregnancy
(C)Lyme disease
(D) All of these - Ans
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